5J26

Crystal structure of a 53BP1 Tudor domain in complex with a ubiquitin variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93.15
Detector technology	IMAGE PLATE
Collection date	2012-10-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	39.531, 47.768, 94.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.593 - 2.505
R-factor	0.237
Rwork	0.231
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.701
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.593	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.071	0.399
Number of reflections	6047
<I/σ(I)>	11.46	1.75
Completeness [%]	87.6	49.1
Redundancy	1.75	1.16
CC(1/2)	0.996	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M Sodium Cacodylate pH 6.0, 0.2 M Sodium Acetate, 27% (w/v) PEG8000