5J22
Crystal structure of Staphylococcal nuclease variant Delta+PHS L103D at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2016-02-17 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.178, 60.353, 38.425 |
Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
Resolution | 38.350 - 1.900 |
R-factor | 0.1803 |
Rwork | 0.177 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.019 |
RMSD bond angle | 1.916 |
Data reduction software | CrysalisPro (1.171.36.32) |
Data scaling software | CrysalisPro (1.171.36.32) |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.018 | 0.113 |
Number of reflections | 11215 | |
<I/σ(I)> | 54.11 | 7.6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.2 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |