5J21
Structure of Bacillus NanoRNase A (WT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.616, 121.250, 123.388 |
| Unit cell angles | 90.00, 91.31, 90.00 |
Refinement procedure
| Resolution | 46.440 - 2.000 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dev |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.072 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.440 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | |
| Number of reflections | 93522 | |
| <I/σ(I)> | 35.19 | |
| Completeness [%] | 98.3 | 96.9 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | PEG MME 2000, sodium acetate, ammonium acetate, glycerol |
