5J1Z
Crystal structure of Staphylococcal nuclease variant Delta+PHS A90D at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2016-03-01 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.178, 60.353, 38.425 |
Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
Resolution | 38.350 - 1.730 |
R-factor | 0.1711 |
Rwork | 0.169 |
R-free | 0.20890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.017 |
RMSD bond angle | 1.763 |
Data reduction software | CrysalisPro (1.171.36.31) |
Data scaling software | CrysalisPro (1.171.36.31) |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.019 | 0.117 |
Number of reflections | 14951 | |
<I/σ(I)> | 49.13 | 6.89 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |