5J1T

TorsinAdeltaE-LULL1 complex, H. sapiens, bound to VHH-BS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	75.435, 88.445, 105.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.733 - 1.402
R-factor	0.1485
Rwork	0.148
R-free	0.17680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.708
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	67.733
High resolution limit [Å]	1.402
Number of reflections	134334
<I/σ(I)>	30.8
Completeness [%]	97.1
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	19 % PEG3350 200 mM ammonium sulfate 100 mM Bis-Tris/HCl pH 6.5