5J1P

Lassa virus L protein cap-snatching endonuclease. Bound to two manganese ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-09-20
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.976
Spacegroup name	P 41 21 2
Unit cell lengths	51.420, 51.420, 144.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.430 - 2.360
R-factor	0.20819
Rwork	0.204
R-free	0.28861
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j1n
RMSD bond length	0.009
RMSD bond angle	1.470
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	2.460
High resolution limit [Å]	2.360	2.360
Rmerge	0.064	1.208
Number of reflections	8543
<I/σ(I)>	22
Completeness [%]	99.8
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	297	2mM MnCl, 5mM DPBA, 5 mM MgCl chloride hexahydrate, 0.05 M HEPES-Na pH7 25 % (v/v) PEG MME 550