5J1M
Crystal structure of Csd1-Csd2 dimer II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.149, 80.013, 74.594 |
| Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.350 |
| R-factor | 0.17103 |
| Rwork | 0.168 |
| R-free | 0.23281 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.395 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 25521 | |
| <I/σ(I)> | 26.6 | 3.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | HEPES, Jeffamine ED-2001 |
