5J1M
Crystal structure of Csd1-Csd2 dimer II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.149, 80.013, 74.594 |
Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.350 |
R-factor | 0.17103 |
Rwork | 0.168 |
R-free | 0.23281 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.395 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 25521 | |
<I/σ(I)> | 26.6 | 3.9 |
Completeness [%] | 99.9 | |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | HEPES, Jeffamine ED-2001 |