5J1L
Crystal structure of Csd1-Csd2 dimer I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.359, 82.375, 76.290 |
Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.270 |
R-factor | 0.17736 |
Rwork | 0.174 |
R-free | 0.23264 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.466 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.310 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 29291 | |
<I/σ(I)> | 20.8 | 4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | HEPES, Jeffamine ED-2001 |