5J1J
Structure of FleN-AMPPNP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-19 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97625 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.050, 206.535, 103.118 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.250 - 1.550 |
R-factor | 0.1707 |
Rwork | 0.170 |
R-free | 0.18910 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.137 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 76475 | |
<I/σ(I)> | 34 | |
Completeness [%] | 99.9 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.26M ammonium sulphate, 0.1M sodium cacodylate |