5J1H

Structure of the spectrin repeats 5 and 6 of the plakin domain of plectin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	105
Detector technology	PIXEL
Collection date	2012-11-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	H 3
Unit cell lengths	178.140, 178.140, 50.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.149 - 2.800
R-factor	0.2247
Rwork	0.223
R-free	0.26930
Structure solution method	SIRAS
RMSD bond length	0.002
RMSD bond angle	0.448
Data reduction software	XDS (November 3, 2014)
Data scaling software	XSCALE (November 3, 2014)
Phasing software	SHELXDE (Version 2013/3)
Refinement software	PHENIX (dev_1931)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.149	2.870
High resolution limit [Å]	2.800	2.800
Rpim	0.122	2.210
Number of reflections	14753
<I/σ(I)>	27.8	2
Completeness [%]	100.0	100
Redundancy	10.2	10.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	Protein at 30 mg/ml in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT was mixed with an equal volume of the crystallization solution 0.2 M Na/K tartrate, 20% PEG3350