5J1F

Structure of the spectrin repeats 5 and 6 of the plakin domain of plectin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-10-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	129.220, 47.760, 106.630
Unit cell angles	90.00, 126.66, 90.00

Refinement procedure

Resolution	43.377 - 3.000
R-factor	0.2464
Rwork	0.245
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1000218819
RMSD bond length	0.002
RMSD bond angle	0.563
Data reduction software	XDS (January 10, 2014)
Data scaling software	XSCALE (January 10, 2014)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX ((dev_2000: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.400	3.080
High resolution limit [Å]	3.000	3.000
Rpim	0.093	0.889
Number of reflections	10510
<I/σ(I)>	15	2
Completeness [%]	97.7	100
Redundancy	3.9	3.9
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	A protein solution at 30 mg/ml in 10 mM Tris-Hcl (pH 7.5), 50 mM NaCl, 1 mM DTT was mixed with an equal volume of crystallization solution 0.1 M Bis-Tris-Propane (pH 6.0), 18% PEG 3350, 0.2 Na malonate