5J1B
structure of the core domaine of Knr4, an intrinsically disordered protein from Saccharomyces cerevisiae - WT.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 62 |
| Unit cell lengths | 103.003, 103.003, 93.377 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 2.500 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22614 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.132 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.250 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.908 | |
| Number of reflections | 19048 | |
| <I/σ(I)> | 12.7 | 1 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 2.6 | 2.6 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | PEG (3000-6000) 15 - 24 % (w/v) pH 8.0 - 9.0 |
