5J14
Crystal structure of endoglycoceramidase I from Rhodococ-cus equi in complex with GM3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-07 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.521, 49.053, 120.135 |
| Unit cell angles | 90.00, 114.19, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.915 |
| Rwork | 0.167 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ccu |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0009) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.915 |
| Rmerge | 0.123 |
| Number of reflections | 79432 |
| <I/σ(I)> | 11 |
| Completeness [%] | 97.4 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 800, sodium hydroxide, ammonium chloride |
