5J10
De novo design of protein homo-oligomers with modular hydrogen bond network-mediated specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.740, 42.120, 123.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.835 - 2.020 |
R-factor | 0.2179 |
Rwork | 0.216 |
R-free | 0.25170 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.653 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1616) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 61.840 |
High resolution limit [Å] | 2.020 |
Number of reflections | 14812 |
<I/σ(I)> | 14.7 |
Completeness [%] | 99.2 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Citric acid pH 3.5, 2.0 M Ammonium sulfate |