5J0T
Binary complex crystal structure of DNA polymerase Beta with G:A mismatch at the primer terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-01 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.400, 79.200, 54.700 |
Unit cell angles | 90.00, 106.10, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.201 |
R-free | 0.25810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3isb |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.112 | 0.467 |
Number of reflections | 30063 | |
<I/σ(I)> | 8.2 | 2.5 |
Completeness [%] | 99.3 | 94.4 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-18% PEG 3350, 50 mM Imidazole, 350 mM Sodium Acetate |