5J0R
Binary complex crystal structure of DNA polymerase Beta with C:A mismatch at the primer terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-30 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.590, 79.490, 55.000 |
Unit cell angles | 90.00, 105.58, 90.00 |
Refinement procedure
Resolution | 19.127 - 2.001 |
R-factor | 0.2147 |
Rwork | 0.208 |
R-free | 0.27240 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.966 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.109 | 0.387 |
Number of reflections | 30340 | |
<I/σ(I)> | 9.2 | 2.76 |
Completeness [%] | 99.2 | 97.6 |
Redundancy | 3.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-18% PEG 3350, 50 mM Imidazole, 350 mM Sodium Acetate |