5J0P

Binary complex crystal structure of DNA polymerase Beta with A:C mismatch at the primer terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-16
Detector	RIGAKU SATURN 92
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	54.140, 79.620, 54.570
Unit cell angles	90.00, 105.55, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.249
R-free	0.31980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bpx
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.079	0.562
Number of reflections	21949
<I/σ(I)>	12.3	2.43
Completeness [%]	96.5	95
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	16-18% PEG 3350,50 mM Imidazole, 350 mM Sodium Acetate