5J0O
Binary complex crystal structure of DNA polymerase Beta with A:A mismatch at the primer terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-03 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.100, 79.500, 54.700 |
| Unit cell angles | 90.00, 105.34, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.210 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpx |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.066 | 0.055 | 0.252 |
| Total number of observations | 107384 | ||
| Number of reflections | 29993 | ||
| <I/σ(I)> | 19.8 | ||
| Completeness [%] | 99.0 | 98.8 | 95.9 |
| Redundancy | 3.6 | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 50 mM Imidazole, pH 7.5, 350 mM Sodium Acetate, 16-18 % PEG3350 |
