5J0H
De novo design of protein homo-oligomers with modular hydrogen bond network-mediated specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 53.194, 53.194, 278.642 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.450 - 1.640 |
R-factor | 0.1808 |
Rwork | 0.178 |
R-free | 0.20710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.600 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1616) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.450 |
High resolution limit [Å] | 1.640 |
Number of reflections | 19278 |
<I/σ(I)> | 10.5 |
Completeness [%] | 100.0 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.06 M Citric acid, 0.04 M BIS-TRIS propane pH 4.1, 16% PEG 3,350 |