5J0G
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form, circular permutant P7/8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-11 |
Detector | MARRESEARCH |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 74.140, 74.140, 59.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.100 - 2.500 |
R-factor | 0.1843 |
Rwork | 0.182 |
R-free | 0.23540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bcz |
RMSD bond length | 0.004 |
RMSD bond angle | 0.881 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.100 | 2.610 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.097 | 0.661 |
Number of reflections | 6519 | |
<I/σ(I)> | 14.1 | |
Completeness [%] | 100.0 | |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M di-ammonium hydrogen citrate, 20 % w/v PEG 3350 |