5J0F
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form, circular permutant P4/5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 71.720, 71.720, 69.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.360 - 1.250 |
R-factor | 0.1313 |
Rwork | 0.129 |
R-free | 0.16960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bcz |
RMSD bond length | 0.022 |
RMSD bond angle | 2.004 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.360 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.039 | 0.563 |
Number of reflections | 56396 | |
<I/σ(I)> | 29.2 | |
Completeness [%] | 100.0 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 0.17 M ammonium sulfate, 25.5 % w/v PEG 4K, 15 % v/v Glycerol |