5J09

Crystal structure of decameric BFDV Capsid Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	77
Detector technology	CCD
Collection date	2015-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	78.791, 148.370, 188.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.994 - 2.000
R-factor	0.21
Rwork	0.208
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.572
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2104: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.990
High resolution limit [Å]	2.000
Number of reflections	148310
<I/σ(I)>	10.7
Completeness [%]	99.2
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.1 M ammonium sulfate and Tris (pH 8.5), 0.1 M Iron (III) chloride hexahydrate