5IZW
Crystal structure of RNA editing specific factor of designer PLS-type PPR-9R protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 41 |
Unit cell lengths | 67.412, 67.412, 56.752 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.501 - 1.738 |
R-factor | 0.1769 |
Rwork | 0.175 |
R-free | 0.20770 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.106 |
Data scaling software | HKL-3000 |
Phasing software | SHELXDE |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.000 |
High resolution limit [Å] | 1.738 |
Number of reflections | 26305 |
<I/σ(I)> | 24.1 |
Completeness [%] | 99.9 |
Redundancy | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Magnesium sulfate heptahydrate, 1,3-Bis[tris(hydroxymethyl)methylamino]propane, PEG 4000 |