5IZS
De novo design of protein homo-oligomers with modular hydrogen bond network-mediated specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 3 |
Unit cell lengths | 72.670, 72.670, 88.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.237 - 2.360 |
R-factor | 0.2465 |
Rwork | 0.245 |
R-free | 0.26880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.628 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.240 | |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.148 | 0.715 |
Number of reflections | 21259 | |
<I/σ(I)> | 12 | 2.4 |
Completeness [%] | 99.0 | 99.8 |
Redundancy | 6.4 | 6.4 |
CC(1/2) | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 278 | 0.2 M Sodium Fluoride, 0.1 M MES pH 5.5 , 20 % PEG 400 |