5IXG
Crystal Structure of Burkholderia cenocepacia BcnB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-11 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.160, 101.170, 63.490 |
Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
Resolution | 37.330 - 1.600 |
R-factor | 0.16189 |
Rwork | 0.160 |
R-free | 0.19448 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wub |
RMSD bond length | 0.024 |
RMSD bond angle | 2.391 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.1.26) |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.330 | 37.300 | 1.630 |
High resolution limit [Å] | 1.600 | 8.470 | 1.600 |
Rmerge | 0.061 | 0.042 | 0.298 |
Number of reflections | 82019 | ||
<I/σ(I)> | 11.3 | ||
Completeness [%] | 97.3 | 70.2 | 78.7 |
Redundancy | 3.8 | 3.2 | 3.1 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | HEPES, PEG 6000 |