5IWZ

Synaptonemal complex protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	65.781, 74.679, 80.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.473 - 2.098
R-factor	0.1929
Rwork	0.190
R-free	0.23890
RMSD bond length	0.008
RMSD bond angle	1.475
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	23639
<I/σ(I)>	20
Completeness [%]	99.9
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M Tris-HCl pH 6.5, 25% PEG 3350