5IWW
Crystal structure of RNA editing factor of designer PLS-type PPR/9R protein in complex with MORF9/RIP9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 64 |
Unit cell lengths | 103.190, 103.190, 149.150 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.595 - 2.650 |
R-factor | 0.1822 |
Rwork | 0.180 |
R-free | 0.22080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.942 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 53.000 |
High resolution limit [Å] | 2.650 |
Number of reflections | 25740 |
<I/σ(I)> | 15.1 |
Completeness [%] | 98.5 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Magnesium sulfate heptahydrate, 1,3-Bis[tris(hydroxymethyl)methylamino]propane, Polyethylene glycol 4000 |