5IWS

Crystal structure of the transporter MalT, the EIIC domain from the maltose-specific phosphotransferase system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97872
Spacegroup name	I 2 2 2
Unit cell lengths	71.633, 108.182, 139.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.832 - 2.551
R-factor	0.2417
Rwork	0.237
R-free	0.28230
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.447
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.610
High resolution limit [Å]	2.550	6.280	2.550
Rmerge	0.123	0.053	0.858
Rmeas	0.130	0.056	0.922
Rpim	0.044	0.018	0.329
Total number of observations	155607
Number of reflections	17976
<I/σ(I)>	7
Completeness [%]	100.0	99.5	100
Redundancy	8.7	9	7.7
CC(1/2)		0.999	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.7	293	39% polyethylene glycol 400, 450 mM NaCl, 3% ethanol, and 100 mM N-(2-Acetamido)iminodiacetic acid