5IWB

Crystal structure of design pentatricopeptide repeat complex with MORF protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	63.110, 63.110, 136.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.410 - 1.764
R-factor	0.18
Rwork	0.179
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.804
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.760
Number of reflections	28002
<I/σ(I)>	40.7
Completeness [%]	99.9
Redundancy	27.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Sodium cacodylate trihydrate,Magnesium acetate tetrahydrate,PEG 8000