5IW7
Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.687, 174.199, 320.474 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.770 - 3.600 |
| R-factor | 0.2689 |
| Rwork | 0.268 |
| R-free | 0.29500 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.770 | 3.800 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.127 | 0.922 |
| Number of reflections | 43821 | |
| <I/σ(I)> | 14.4 | 2.9 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 10 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 8-14% w/v PEG 3350 0.18-0.30 M sodium malonate pH 6.0 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 8-14% w/v PEG 3350 0.18-0.30 M sodium malonate pH 6.0 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 8-14% w/v PEG 3350 0.18-0.30 M sodium malonate pH 6.0 |
