5IW7

Crystal structure of yeast Tsr1, a pre-40S ribosome synthesis factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	65.687, 174.199, 320.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.770 - 3.600
R-factor	0.2689
Rwork	0.268
R-free	0.29500
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	3.800
High resolution limit [Å]	3.600	3.600
Rmerge	0.127	0.922
Number of reflections	43821
<I/σ(I)>	14.4	2.9
Completeness [%]	99.9	99.5
Redundancy	10	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	8-14% w/v PEG 3350 0.18-0.30 M sodium malonate pH 6.0
1	VAPOR DIFFUSION, SITTING DROP	6	291	8-14% w/v PEG 3350 0.18-0.30 M sodium malonate pH 6.0
1	VAPOR DIFFUSION, SITTING DROP	6	291	8-14% w/v PEG 3350 0.18-0.30 M sodium malonate pH 6.0