5IVO

BC2 nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.918409
Spacegroup name	P 21 21 21
Unit cell lengths	31.630, 47.550, 67.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.900 - 1.800
R-factor	0.191
Rwork	0.187
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x1o
RMSD bond length	0.014
RMSD bond angle	1.414
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.900	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	9931
<I/σ(I)>	17.14
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	MES/imidazole, 2-Methyl-2,4-pentanediol (MPD), PEG 1000, PEG 3350, 1,6-hexanediol, 1-butanol, (RS)-1,2- propanediol, 2-propanol, 1,4-butanediol, 1,3-propanediol