5IVN
BC2 nanobody in complex with the BC2 peptide tag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.010, 31.530, 35.880 |
| Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
| Resolution | 50.550 - 1.000 |
| R-factor | 0.12908 |
| Rwork | 0.128 |
| R-free | 0.14961 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ivo |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.549 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.550 | 1.030 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Number of reflections | 61121 | |
| <I/σ(I)> | 18.42 | |
| Completeness [%] | 99.6 | |
| Redundancy | 9.5 | |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES/imidazole, 2-Methyl-2,4-pentanediol, PEG 1000, PEG 3350, DL-Glutamatic acid monohydrate, DL-Alanine, Glycine, DL-Lysine monohydrochloride, DL-Serine |
