5IV9

The LPS Transporter LptDE from Klebsiella pneumoniae, full-length

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	90
Detector technology	PIXEL
Collection date	2013-06-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	131.219, 155.459, 197.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.717 - 4.369
R-factor	0.3035
Rwork	0.301
R-free	0.34370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.631
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.717
High resolution limit [Å]	4.369
Rmerge	0.099
Number of reflections	12420
<I/σ(I)>	14.3
Completeness [%]	95.6
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	294	25% 1,2-propanediol, 100 mM phosphate-citrate pH 4.2, 10% glycerol, 5% PEG 3000