5IV9
The LPS Transporter LptDE from Klebsiella pneumoniae, full-length
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2013-06-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 131.219, 155.459, 197.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.717 - 4.369 |
| R-factor | 0.3035 |
| Rwork | 0.301 |
| R-free | 0.34370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.631 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.717 |
| High resolution limit [Å] | 4.369 |
| Rmerge | 0.099 |
| Number of reflections | 12420 |
| <I/σ(I)> | 14.3 |
| Completeness [%] | 95.6 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 294 | 25% 1,2-propanediol, 100 mM phosphate-citrate pH 4.2, 10% glycerol, 5% PEG 3000 |
