5IUM
Crystal structure of phosphorylated DesKC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 94.443, 94.443, 161.848 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.499 - 3.162 |
| R-factor | 0.259 |
| Rwork | 0.257 |
| R-free | 0.29500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3gig |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data scaling software | XDS (Jun 17, 2015) |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.499 | 3.173 |
| High resolution limit [Å] | 3.162 | 3.162 |
| Number of reflections | 14829 | |
| <I/σ(I)> | 23.7 | 2.1 |
| Completeness [%] | 100.0 | 94.1 |
| Redundancy | 7.2 | 6.1 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 291 | PEG3000, MgCl2, CHES, AMP-PCP, pH 9.5, vapor diffusion, hanging drop, temperature 291K |
