5IUJ
Crystal structure of the DesK-DesR complex in the phosphotransfer state with low Mg2+ (20 mM)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.980100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.820, 114.619, 91.597 |
Unit cell angles | 90.00, 116.44, 90.00 |
Refinement procedure
Resolution | 66.698 - 3.200 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.260 |
Data scaling software | XDS (Jun 17, 2015) |
Phasing software | PHASER (2.5.7) |
Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.698 | 3.207 |
High resolution limit [Å] | 3.196 | 3.196 |
Rmerge | 0.110 | 0.731 |
Number of reflections | 26369 | |
<I/σ(I)> | 7.7 | 1.8 |
Completeness [%] | 97.4 | 96.8 |
Redundancy | 2.4 | 2.4 |
CC(1/2) | 0.991 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG3350, tri-potassium citrate, vapor diffusion, hanging drop, temperature 291K |