5IUD
Human DNA polymerase alpha in binary complex with a DNA:DNA template-primer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 137.040, 132.030, 163.139 |
| Unit cell angles | 90.00, 109.13, 90.00 |
Refinement procedure
| Resolution | 64.736 - 3.300 |
| R-factor | 0.1854 |
| Rwork | 0.183 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.480 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.2.1) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 86.220 | 86.220 | 3.370 |
| High resolution limit [Å] | 3.300 | 16.830 | 3.300 |
| Rmerge | 0.083 | 0.063 | 0.761 |
| Rmeas | 0.100 | 0.077 | 0.935 |
| Rpim | 0.056 | 0.045 | 0.535 |
| Total number of observations | 248849 | 1870 | 13028 |
| Number of reflections | 82602 | ||
| <I/σ(I)> | 7.8 | 20.7 | 1.5 |
| Completeness [%] | 99.6 | 98.9 | 99.4 |
| Redundancy | 3 | 2.8 | 2.8 |
| CC(1/2) | 0.989 | 0.927 | 0.548 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 10% polyethylene glycol 4000, 0.2M sodium citrate, 10% isopropanol, 0.1M sodium citrate buffer (pH = 5.5) |
