5IUD

Human DNA polymerase alpha in binary complex with a DNA:DNA template-primer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	137.040, 132.030, 163.139
Unit cell angles	90.00, 109.13, 90.00

Refinement procedure

Resolution	64.736 - 3.300
R-factor	0.1854
Rwork	0.183
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	MOSFLM
Data scaling software	Aimless (0.2.1)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.220	86.220	3.370
High resolution limit [Å]	3.300	16.830	3.300
Rmerge	0.083	0.063	0.761
Rmeas	0.100	0.077	0.935
Rpim	0.056	0.045	0.535
Total number of observations	248849	1870	13028
Number of reflections	82602
<I/σ(I)>	7.8	20.7	1.5
Completeness [%]	99.6	98.9	99.4
Redundancy	3	2.8	2.8
CC(1/2)	0.989	0.927	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	10% polyethylene glycol 4000, 0.2M sodium citrate, 10% isopropanol, 0.1M sodium citrate buffer (pH = 5.5)