5ITS
Crystal structure of LOG from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 113.506, 130.501, 140.512 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.620 - 2.300 |
| R-factor | 0.1775 |
| Rwork | 0.175 |
| R-free | 0.22780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a33 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.704 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.620 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.102 | 0.320 |
| Number of reflections | 44133 | |
| <I/σ(I)> | 17.5 | 3.5 |
| Completeness [%] | 95.2 | 87 |
| Redundancy | 6.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 3350, DL-malic acid |
