5ITM
The structure of truncated histone-like protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 123 |
Detector technology | CCD |
Collection date | 2015-07-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9760 |
Spacegroup name | P 31 |
Unit cell lengths | 49.333, 49.333, 82.466 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.490 - 1.400 |
R-factor | 0.1696 |
Rwork | 0.168 |
R-free | 0.19740 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.301 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 43748 | |
<I/σ(I)> | 30.6 | |
Completeness [%] | 99.2 | |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M Tris-Cl, pH 7.5, 40% PEG 400 |