5ITJ

The structure of histone-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-09-29
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	41.464, 48.538, 55.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.630
R-factor	0.18099
Rwork	0.178
R-free	0.22875
RMSD bond length	0.024
RMSD bond angle	2.639
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.690
High resolution limit [Å]	1.630	1.630
Number of reflections	14365
<I/σ(I)>	44.7
Completeness [%]	97.7
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	295	0.1 M Tris-Cl, 40% PEG 400, 0.2 M Li2SO4