5IT8
High-resolution structure of the Escherichia coli ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 211.920, 434.360, 623.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.194 - 3.120 |
Rwork | 0.190 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ybb |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.194 | 8.940 | 3.380 |
High resolution limit [Å] | 3.117 | 6.320 | 3.160 |
Rmerge | 0.122 | 0.083 | 0.332 |
<I/σ(I)> | 82.3 | 18.6 | 7.77 |
Completeness [%] | 89.4 | 99.4 | 99.3 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD |