5IT6

Galectin-related protein: an integral member of the network of chicken galectins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	I 21 21 21
Unit cell lengths	38.584, 106.865, 114.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.044 - 1.547
R-factor	0.172
Rwork	0.171
R-free	0.18820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b9c
RMSD bond length	0.008
RMSD bond angle	1.036
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	77.993	57.043	1.630
High resolution limit [Å]	1.547	4.890	1.550
Rmerge		0.042	0.977
Rmeas	0.074
Rpim	0.025
Total number of observations	300868
Number of reflections	34844
<I/σ(I)>	12.6	13.8	0.8
Completeness [%]	99.7	99.9	97.6
Redundancy	8.6	8	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	2.0 M (NH4)2SO4, 100 mM MES (pH 6.5) and 5% (w/v) PEG 400