5IT3
Swirm domain of human Lsd1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2005-08-24 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9777, 0.979, 0.9793, 0.9649 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.170, 49.970, 48.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.850 - 1.400 |
| R-factor | 0.16108 |
| Rwork | 0.159 |
| R-free | 0.19405 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.473 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 28163 |
| <I/σ(I)> | 4.3 |
| Completeness [%] | 99.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 0.1 M MgCl2, 0.1 M Tris, 25%(w/v) PEG 3350 |
