5ISU
2.2 Angstrom Crystal Structure of ABC Transporter Substrate Binding Protein CtaP (Lmo0135) from Listeria monocytogenes.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.637, 75.160, 70.517 |
Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
Resolution | 29.100 - 2.200 |
R-factor | 0.18005 |
Rwork | 0.178 |
R-free | 0.22315 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oet |
RMSD bond length | 0.009 |
RMSD bond angle | 1.434 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.100 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.050 | 0.756 |
Number of reflections | 24855 | |
<I/σ(I)> | 24.5 | 2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.629 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | Protein: 15.0 mg/ml, 0.1M Tris HCl (pH 8.3), 0.5mM Cysteine; Screen: Classics II (D8), 0.1M HEPES (pH 7.5), 25% (w/v) PEG3350. |