5IS7

Crystal structure of mouse CARM1 in complex with decarboxylated SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9840
Spacegroup name	P 21 21 2
Unit cell lengths	74.528, 98.935, 206.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.110 - 2.290
R-factor	0.2107
Rwork	0.209
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.002
RMSD bond angle	0.656
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1980)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.110	2.340
High resolution limit [Å]	2.290	2.290
Rmerge	0.086	0.200
Number of reflections	69754
<I/σ(I)>	10.2	0.8
Completeness [%]	99.5	93.7
Redundancy	6.4	6.1
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	297	Tris-HCl pH 8.0 100 mM PEG 2000 MME 25% NaCl 167 mM