5IS1
Crystal structure of the extracellular domain of sensor histidine kinase YycG from Staphylococcus aureus at 2.0 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-11 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 50.610, 50.610, 93.145 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.829 - 1.998 |
R-factor | 0.206 |
Rwork | 0.203 |
R-free | 0.22860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.385 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.070 |
High resolution limit [Å] | 1.998 | 4.300 | 2.000 |
Rmerge | 0.052 | 0.045 | 0.331 |
Number of reflections | 9779 | ||
<I/σ(I)> | 71.355 | ||
Completeness [%] | 99.4 | 94.5 | 100 |
Redundancy | 10.3 | 8.7 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6 | 295 | 40-55% (w/v) PEG 400, 100mM 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.0 |