5IS1
Crystal structure of the extracellular domain of sensor histidine kinase YycG from Staphylococcus aureus at 2.0 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.610, 50.610, 93.145 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.829 - 1.998 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.385 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.070 |
| High resolution limit [Å] | 1.998 | 4.300 | 2.000 |
| Rmerge | 0.052 | 0.045 | 0.331 |
| Number of reflections | 9779 | ||
| <I/σ(I)> | 71.355 | ||
| Completeness [%] | 99.4 | 94.5 | 100 |
| Redundancy | 10.3 | 8.7 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 295 | 40-55% (w/v) PEG 400, 100mM 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.0 |
