5IRS
crystal structure of the proteasomal Rpn13 PRU-domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.555, 56.476, 64.089 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.451 - 1.796 |
R-factor | 0.1713 |
Rwork | 0.167 |
R-free | 0.20920 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2r2y |
RMSD bond length | 0.019 |
RMSD bond angle | 1.501 |
Data reduction software | HKL-2000 (HKL-2000) |
Data scaling software | HKL-2000 (HKL-2000) |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1218) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.796 | 1.800 |
Rmerge | 0.066 | 0.960 |
Number of reflections | 14838 | |
<I/σ(I)> | 15.72 | 1.82 |
Completeness [%] | 99.1 | 96.35 |
Redundancy | 6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG4000, Sodium acetate |