5IRR
Crystal structure of Septin GTPase domain from Chlamydomonas reinhardtii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-26 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9200 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.580, 39.410, 130.900 |
Unit cell angles | 90.00, 118.50, 90.00 |
Refinement procedure
Resolution | 43.628 - 2.040 |
R-factor | 0.1964 |
Rwork | 0.195 |
R-free | 0.23220 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.639 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHELX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.630 | 43.630 | 2.090 |
High resolution limit [Å] | 2.040 | 9.120 | 2.040 |
Rmerge | 0.086 | 0.040 | 0.729 |
Number of reflections | 49936 | ||
<I/σ(I)> | 11.4 | ||
Completeness [%] | 97.1 | 98 | 95.9 |
Redundancy | 4.1 | 3.9 | 3.9 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD; 30mM of each sodium nitrate, disodium hydrogen phosphate, ammonium sulfate; 10mM bicine/Trizma base pH 8.5 |