5IRF
Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P1 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 50.290, 79.160, 86.020 |
Unit cell angles | 109.00, 90.56, 105.04 |
Refinement procedure
Resolution | 46.470 - 1.600 |
R-factor | 0.1924 |
Rwork | 0.191 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hhj |
RMSD bond length | 0.006 |
RMSD bond angle | 0.784 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2254: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.470 |
High resolution limit [Å] | 1.600 |
Number of reflections | 152938 |
<I/σ(I)> | 19.3 |
Completeness [%] | 96.1 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 100 mM Bis-Tris propane pH 8.5, 200 mM NaOAc-3H2O, 20% PEG 3350 |