5IRB
Structural insight into host cell surface retention of a 1.5-MDa bacterial ice-binding adhesin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-11-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.078 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.601, 247.023, 84.255 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.789 - 2.000 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.510 | 2.030 |
| High resolution limit [Å] | 1.800 | 2.000 |
| Rmerge | 0.081 | |
| Number of reflections | 60756 | |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 8.2 | |
| CC(1/2) | 0.996 | 0.467 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 298 | PEG3350, calcium chloride |
