5IQZ

Crystal structure of N-terminal domain of Human SIRT7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE BL-21
Synchrotron site	RRCAT INDUS-2
Beamline	BL-21
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97947
Spacegroup name	P 1 21 1
Unit cell lengths	60.490, 49.330, 65.680
Unit cell angles	90.00, 101.20, 90.00

Refinement procedure

Resolution	48.605 - 2.334
R-factor	0.1856
Rwork	0.183
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf
RMSD bond length	0.002
RMSD bond angle	0.644
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.429	64.429	2.460
High resolution limit [Å]	2.334	7.380	2.334
Rmerge		0.082	0.391
Number of reflections	16201
<I/σ(I)>	8.1	6.4	1.9
Completeness [%]	98.8	99.6	96.4
Redundancy	3.7	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1M Sodium cacodylate, pH 5.5, 25% w/v PEG 4000